ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

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Such powder-free films might have a different kind of a problem. Some of the proteins can be more harmful than others. Two different sources of blood s5712 were used.

Removing latex proteins with fumed silica additives | Rubber and Plastics News

However, the 20 ppm proteins left, may happen to be harmful and the latex status as far as allergen content did not change at all. Tables I and II report results with silica at a level of 1 to 5 weight percentage. Further analysis by the LEAP method also at Guthrie Research, resulted in clean films having protein values below the detection level, 0. Some laboratories claim that they can measure lower levels of proteins.

The proteins then easily are removed.

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Historically latex gloves are powdered for two reasons. Powder-free latex gloves and other methods of reducing protein content Latex gloves are among the most commonly used critical latex articles. While donning and removing powdered gloves, the protein-coated particles become air borne dust and can contaminate large volumes.


Latex d57122 without silica, does have protein residues, see Table II. The qstm probably would stay adsorbed on the latex particles throughout the compounding process. The test method involves an extraction and precipitation procedure followed by an assay of protein content. D Specification for Rubber Examination Gloves.

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Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. Register for a trial account. Hamilton, Journal d712 Allergy and Clinical Immunology, 96, For specific hazard statements, see Section 9.

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Contrary to conventional thickening agents, silica enhances the tensile. It is expected that work will be performed in a properly equipped laboratory.

A previous paper on cleaning latex films with fumed silica5 presented protein results derived by ASTM D Remember me for one month. The silica also may serve as a thickening agent. That’ll take 30 years For autonomous vehicles to gain passengers’ trust, communication is key ‘Impatient’ Mary Barra reawakens spirit of crisis at GM Nitto invests to understand, better serve its customers Self-driving cars might make people sick to their stomachs.

This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. Allergen units can range from 10, units per ml for an extract from raw latex film to 1 unit per ml extracted from vinyl glove film negative control or latex with undetectable level of allergenic proteins. This is in contradiction to what was proposed before where fumed silica attaches itself to the proteins on the surface of the latex particle, binding them there.


However, these methods are good for the coated surface only and proteins migrate to both sides. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. It is suggested that the silica attaches itself to the surface of the latex particle substituting the protein molecules and displacing them, see Fig. Other methods for protein removal treatment include passing the latex through special ion exchange resins6 or altering the protein structure with protease enzymes.

Historical Version s – view previous versions of standard. For example, someone can develop a process for latex protein cleaning, submit the clean latex to ASTM D test and find that the protein level was reduced from ppm to 20 ppm.

The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard. D Specification for Rubber Surgical Gloves. D57112 book title Enter keywords for book title search.